Method for the determination of sugars in invert sugar by refractive index method - Master's thesis - Dissertation

Summary

Objective: To determine the content of sugars in invert sugar. METHODS: The content of glucose and fructose in invert sugar and ointment was determined by refractive rotation method. Conclusion: This method is simple, accurate and reproducible, and can be used for the determination of sugar content in invert sugar and ointment.

Key words: invert sugar, fructose, glucose, refractive index method Determination of sugars is an important pharmaceutical preparation auxiliary, the Chinese Pharmacopoeia contains glucose as a nutrient, and sucrose is used as a flavoring agent commonly used in pharmaceutical preparations. Sucrose is a disaccharide formed by the polymerization of a molecule of glucose and a molecule of fructose. Its crystallinity makes it present in a liquid or semi-solid preparation such as ointment at a higher concentration. It is easy to re-precipitate the crystal during storage and affect the uniformity of the preparation. Stability, in order to overcome crystallization, improve sweetness and oxidation resistance, and facilitate the shaping, sucrose is dissolved in a small amount of water, in the presence of 0.1% tartaric acid, at 110-115 ° C for 2 hours to make invert sugar, conversion Like honey, sugar is primarily a mixture of glucose and fructose, which many companies use instead of sucrose, honey for medicine and the food industry. In order to control the quality of invert sugar and its preparation, we used the Repo-3 invert sugar conversion optical rotation machine to determine the content of glucose and fructose in invert sugar and ointment, and we can evaluate the conversion sugar in the conversion process and the finished product stage. Conversion rate. The sucrose and honey in the ointment must be added after refining, the purpose of which is to remove impurities, kill microorganisms, reduce moisture, and prevent the "returning sand" of the ointment (the crystallization of sugar after a certain time of storage of the ointment). The method of refining sugar is: taking sucrose and adding half of the sugar and 0.1% of tartaric acid, heating and dissolving to maintain a slight boiling, until the sugar liquid is golden yellow, and the conversion rate is 40% to 50%.
The amount of sugar contained in the sand and the ointment is related to the invert sugar. The total sugar content is controlled below 85%, and the conversion rate is controlled at 40-50%. The Repo-3 invert sugar refraction optical rotation machine quickly measures the content of various sugars in the ointment, including fructose, glucose and sucrose.

The content of invert sugar in ointment

Conclusion of the influence of the refractive index of glucose in invert sugar on ointment

The conventional quantitative method is quantitatively determined by a redox titration method or a chromatographic method using properties such as reduction properties of a saccharide, high performance liquid chromatography, and the like, and is a ratio of a saccharide content present in a mixed state and a pharmaceutical agent. It is susceptible to interference and reduces the accuracy of the measurement. The use of refractive optical rotation method Repo-3 invert sugar conversion optical rotation machine is more suitable for the determination of sugar content and determination of sugar ratio in mixed state. ATAGO refractometer and calibration standard solution for sugar meter

ATAGO Refractometers and Brix meters correct all products before shipment, and there will be no deviation from the scale unless an accident occurs during shipment. However, if there is a deviation after a long period of use or after an external impact, the zero point needs to be corrected. For refractometers that cannot be zeroed with water, use a standard to calibrate the zero point.