Laboratory kit operation correct solution - Database & Sql Blog Articles

The kit is too long in transit and the temperature is too high. Try to shorten the transportation time, and cool the ice in summer. The kit is not well balanced. Remove the kit from the 2 to 8 ° C refrigerator, open the lid, and equilibrate for at least 20 minutes at room temperature to ensure that all reagents are equilibrated to room temperature (approximately 25 ° C). The incubator temperature is less than 37 °C. Pay attention to the temperature of the incubator, and reduce the number of opening times after putting it into the reaction plate to avoid affecting the constant temperature. Especially, the non-water-blocking incubator should pay attention to it. Insufficient holding time. Correct the timing of the fixed clock. The impact force during washing is too large, the soaking time is too long, and the number of washings is increased. Keep the washing time as required by the instructions and accurately remember the number of washings. The pipette has insufficient liquid absorption, too much water is hanged on the inner wall of the nozzle or the inner wall is not clean. Correct the pipette, the nozzle should be matched, the nozzle should be tight when it is installed, and the inner wall of the nozzle should be clean. It is best to use it once. There is a problem with the quality of distilled water. Use fresh, qualified distilled water. The substrate has insufficient action time. Accurate timing.